Generator

Part:BBa_K4628028:Design

Designed by: Qinyao Yan   Group: iGEM23_ZJU-China   (2023-10-11)


Pikm-1(Enhancer) expression circuit


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1850
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 3973
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3079
    Illegal BamHI site found at 2704
    Illegal XhoI site found at 3537
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1850
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1850
    Illegal SpeI site found at 2424
    Illegal SpeI site found at 2430
    Illegal PstI site found at 2643
    Illegal PstI site found at 2859
    Illegal PstI site found at 2874
    Illegal PstI site found at 2943
    Illegal PstI site found at 3387
    Illegal NgoMIV site found at 1075
    Illegal NgoMIV site found at 1090
    Illegal NgoMIV site found at 2458
    Illegal NgoMIV site found at 3555
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2688
    Illegal SapI site found at 1626
    Illegal SapI.rc site found at 885
    Illegal SapI.rc site found at 3596


Design Notes

By modifying the recognition region of NLR with nanoantibodies, the targeting and recognition spectrum of immune receptors have the ability to be artificially engineered, providing a new approach for plant disease resistance.


Source

Combined with CaMV 35S promoter, Pikm-1-Enhancer, CaMV poly(A) signal

References